Wound healing is an essential biological process for maintaining homeostasis and, ultimately, for survival. We investigate the mechanisms underlying extreme wound healing in Stentor coeruleus, a single-celled organism, capable of recovering from drastic membrane wounds exceeding half of the cell surface. This talk focuses on our recent effort on developing a microfluidic platform for the manipulation and reproducible wounding of the cell. We demonstrate a microfluidic “guillotine” for bisecting cells in a continuous flow, and a “SMORES” platform to immobilize the cells for laser ablation. We further discuss the extension of the tool for dissecting live tumors to generate organoids that preserve the tumor immune microenvironment for applications including the testing of immunotherapy on a chip.