Manipulation of particles in micro and nano scale has been invaluable in a variety of scenarios in applied physics, chemistry and biomedicine. Acoustic microfluidics has emerged as a powerful and novel platform for micro/nano manipulation in many applications recently due to its advantages in versatility, low cost, easy to integrate and manufacture, miniaturization, energy efficiency, etc.. Here, I will first introduce the development of acoustic tweezer which can manipulation objects from nano to mm scale on a chip. I will then report our most recent breakthroughs on acoustic manipulation, including (1) acoustic thermal shift assay for protein manipulation and characterization, (2) internal structure manipulation of a novel endoskeletal droplet using acoustic waves, and (3) staged assembly of colloids.