CRISPR is a gene editing technique that allows researchers to quickly and precisely edit genome sequences. Beyond gene editing, CRISPR-Cas systems can be repurposed as a programmable platform for other various applications. In this talk, I will introduce how we use CRISPR technology to program microfluidic devices for DNA detection, DNA computing and digital display. First, I will present our advancements in investigating simple, sensitive and specific CRISPR assay technologies for DNA detection. Second, I will discuss how to integrate CRISPR assay with microfluidics technology to develop affordable and programmable microfluidic diagnostic systems for infectious disease detection and cancer diagnostics. Third, I will describe how we program CRISPR and DNA to develop paper-based microfluidic devices for DNA computing and digital display. Overall, by combining the programmability of CRISPR with the integration capability of microfluidics, we can develop next-generation tools not only for biomedical diagnostics, but also for information technology.