BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Penn Engineering Events - ECPv6.15.19//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-WR-CALNAME:Penn Engineering Events
X-ORIGINAL-URL:https://seasevents.nmsdev7.com
X-WR-CALDESC:Events for Penn Engineering Events
REFRESH-INTERVAL;VALUE=DURATION:PT1H
X-Robots-Tag:noindex
X-PUBLISHED-TTL:PT1H
BEGIN:VTIMEZONE
TZID:America/New_York
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20190310T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20191103T060000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20200308T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20201101T060000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20210314T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20211107T060000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20200128T120000
DTEND;TZID=America/New_York:20200128T130000
DTSTAMP:20260408T031740
CREATED:20200121T211501Z
LAST-MODIFIED:20200121T211501Z
UID:2534-1580212800-1580216400@seasevents.nmsdev7.com
SUMMARY:MEAM Doctoral Dissertation Defense: "Applications of a Double-stranded Elastic Rod Model to DNA"
DESCRIPTION:In the first part of the presentation\, we discuss a double-stranded elastic rod (birod) model and apply it to study allosteric interactions between two ligands on DNA. Next\, we combine the birod model with statistical mechanics and use it to study the temperature-induced strand separation in dsDNA. \nAllosteric interaction between two ligands on DNA is quantified by measuring the change in free energy of the DNA complex (DNA + two ligands) as a function of the distance between the binding sites for two ligands. We show that trends in the interaction energy of two ligands binding to DNA can be explained using the birod model which accounts for the helical shape of DNA\, elastic deformation of strands and base-pairs\, and the stacking energy due to perturbations in position and orientation of the bases caused by the binding of ligands. The model predicts that the allosteric interaction energy between two ligands decays exponentially with the distance between them and oscillates with the periodicity of the double helix\, which by appropriate parameter fitting is shown to quantitatively match with the experimental measurements. Our model predicts an identical trend (exponentially decaying sinusoid) in the perturbation of groove width produced by the binding of a single ligand—consistent with the published results from molecular simulations. Our analysis provides a new framework to understand allosteric interactions in DNA and can be extended to other rod-like macromolecules whose elasticity plays a key role in their biological functions. \nIn the next part of the presentation\, we combine statistical mechanics with continuum mechanics to predict the mechanical response of DNA molecules. We observe that the external force and torque driven microstructure evolution in DNA leads to force-extension curves that exhibit strong signatures of first-order phase transitions. The effects of the electrostatic interactions on the mechanical-response predicted from the model resolve some important counter-intuitive experimental observations. Next\, we focus on one such structural transition—temperature-driven disintegration of dsDNA into two single strands\, known as DNA melting. We illustrate how the continuum mechanics of birod coupled with statistical mechanics can be used to study the micromechanics of DNA melting. We note that a nonlinear asymmetric interaction between the outer strands leads to a sudden and highly cooperative melting transition. The model enables us to couple the effect of tensile force on the melting temperature\, which to the author’s knowledge is the first mechanics-based attempt to do so. The results from the models\, after suitably choosing the parameter values\, agree with multiple experiments reported in the literature and they generate new falsifiable predictions that can be experimentally tested.
URL:https://seasevents.nmsdev7.com/event/meam-doctoral-dissertation-defense-applications-of-a-double-stranded-elastic-rod-model-to-dna/
LOCATION:4E9\, DRLB\, 209 S. 33rd Street\, Philadelphia\, PA\, 19104\, United States
CATEGORIES:Seminar,Doctoral,Student,Dissertation or Thesis Defense
ORGANIZER;CN="Mechanical Engineering and Applied Mechanics":MAILTO:meam@seas.upenn.edu
END:VEVENT
END:VCALENDAR