BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Penn Engineering Events - ECPv6.15.18//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-WR-CALNAME:Penn Engineering Events
X-ORIGINAL-URL:https://seasevents.nmsdev7.com
X-WR-CALDESC:Events for Penn Engineering Events
REFRESH-INTERVAL;VALUE=DURATION:PT1H
X-Robots-Tag:noindex
X-PUBLISHED-TTL:PT1H
BEGIN:VTIMEZONE
TZID:America/New_York
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20200308T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20201101T060000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20210314T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20211107T060000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20220313T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20221106T060000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20210224T150000
DTEND;TZID=America/New_York:20210224T160000
DTSTAMP:20260407T035712
CREATED:20210122T015650Z
LAST-MODIFIED:20210122T015650Z
UID:4000-1614178800-1614182400@seasevents.nmsdev7.com
SUMMARY:CBE Seminar: "Probing Protein Interactions from Molecular to Cellular Scales with Microscale Technologies"
DESCRIPTION:Abstract \nBiological molecules rarely act alone. For example\, in many pediatric cancers\, a chromosomal rearrangement results in a fusion protein with altered DNA and protein interactions that yield proliferative and aggressively metastatic cell subpopulations. In this talk\, I will describe quantification of molecular interaction properties relevant to fusion oncoprotein biology using microscale tools with distinct forces tailored to each biological question. First\, I will discuss measurement of cellular heterogeneity in cytoskeletal protein complex abundance as is needed to uncover ‘rogue’ metastatic and drug resistant cells. I introduced a microfluidic biochemical assay using electrophoretic forces and sieving hydrogels to fractionate dynamic protein complexes from single cells faster than dissociation kinetics. Second\, I will describe biophysically probing protein-DNA molecular mechanics to understand how tension plays a role in DNA pairing during DNA damage repair. To reveal forces the Rad51 repair protein exerts on DNA\, microscale tweezers apply a magnetic piconewton-scale force to single molecules of DNA. Finally\, I will share future directions discerning the biochemical and biophysical roles of multi-component protein complexes in fusion oncoprotein-driven pediatric cancers\, such as Ewing’s sarcoma. My group will uncover new targets for ‘molecularly surgical’ Ewing’s sarcoma therapies through interrogation of molecular interactions towards replacing combination chemotherapy and crude tumor removal surgeries.
URL:https://seasevents.nmsdev7.com/event/cbe-seminar-probing-protein-interactions-from-molecular-to-cellular-scales-with-microscale-technologies/
LOCATION:Zoom – Email CBE for link
CATEGORIES:Seminar
ORGANIZER;CN="Chemical and Biomolecular Engineering":MAILTO:cbemail@seas.upenn.edu
END:VEVENT
END:VCALENDAR