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DTSTART;TZID=America/New_York:20250924T153000
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DTSTAMP:20260602T010119
CREATED:20250912T211221Z
LAST-MODIFIED:20250912T211221Z
UID:20972-1758727800-1758731400@seasevents.nmsdev7.com
SUMMARY:CBE Seminar: "Imaging Genetics: Enhancer-mediated Dynamic Gene Control in Space and Time" (Bomyi Lim\, University of Pennsylvania)
DESCRIPTION:Abstract: \nCanonical models suggest that regulatory enhancers function independently of genomic distance\, position\, and orientation to activate the target gene. Indeed\, a wide range of genomic distances and relative enhancer positions exist in endogenous contexts. However\, systematic analysis of how specific enhancer-promoter configurations affect transcriptional dynamics has been limited by technical constraints. Here\, we employ single-cell MS2/MCP-based live imaging in Drosophila embryos to systematically analyze transcriptional dynamics driven by varying enhancer-promoter configurations. Our analyses reveal that while linear enhancer-promoter distance moderately tunes transcriptional output\, downstream enhancer positioning relative to the promoter reduces mRNA output by 70%. Each configuration modulates distinct transcriptional parameters: linear distance governs initiation kinetics\, while relative enhancer positioning dictates transcriptional stability. Although the downstream enhancer positioning destabilized the enhancer-promoter interactions\, transcription factor hub properties remained comparable between two configurations\, revealing a clear dissociation between TF hub dynamics and E-P configuration-dependent transcriptional activity. These effects are consistent across varied enhancer and reporter sequences\, revealing configuration-dependent cis-regulatory element arrangement as an intrinsic mechanism for transcriptional fine-tuning. This work challenges the paradigm of configuration-independent enhancer function and establishes a framework to dissect the interplay between genome architecture and trans-acting factors.
URL:https://seasevents.nmsdev7.com/event/cbe-seminar-imaging-genetics-enhancer-mediated-dynamic-gene-control-in-space-and-time-bomyi-lim-university-of-pennsylvania/
LOCATION:Wu and Chen Auditorium (Room 101)\, Levine Hall\, 3330 Walnut Street\, Philadelphia\, PA\, 19104\, United States
CATEGORIES:Seminar,Faculty
ORGANIZER;CN="Chemical and Biomolecular Engineering":MAILTO:cbemail@seas.upenn.edu
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