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DTSTART;TZID=America/New_York:20220407T153000
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CREATED:20211210T164738Z
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UID:5933-1649345400-1649349000@seasevents.nmsdev7.com
SUMMARY:BE Seminar: "Analysis of High-content Genomic Screening Data with Large-scale Optical Pooled Screens" (Paul Blainey\, MIT)
DESCRIPTION:This seminar will be held in person in Towne 337 and on zoom (check email for link or contact ksas@seas.upenn.edu). \nGenetic screens are critical for the systematic identification of genes underlying cellular phenotypes. While pooling gene perturbations greatly increases screening throughput\, this approach was not yet compatible with the high-content imaging of complex and dynamic cellular phenotypes. Our group recently developed optical pooled screening using in situ sequencing-by-synthesis to link pooled perturbations with their associated visual phenotypic outcomes at the single-cell level in mammalian cells. We have since established this approach for very large-scale screens where hundreds of phenotypic parameters are recorded for each cell.  I will provide an overview of the applicability of this kind of approach and discuss in more detail two large-scale\, high-content collaborative screening projects\, one related to intracellular responses to viral infection\, and another focused on human cell division phenotypes.
URL:https://seasevents.nmsdev7.com/event/be-seminar-paul-blainey/
LOCATION:Room 337\, Towne Building\, 220 South 33rd Street\, Philadelphia\, PA\, 19104\, United States
CATEGORIES:Seminar
ORGANIZER;CN="Bioengineering":MAILTO:be@seas.upenn.edu
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