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SUMMARY:BE Seminar: "Imaging and sequencing single cells" (Aaron Streets)
DESCRIPTION:This event will be held virtually on zoom. Check your email for the link and passcode or contact ksas@seas.upenn.edu. \nRecent advances in microfluidics and high-throughput sequencing technology have enabled rapid profiling of genomic material in single cells. Valve- and droplet-based microfluidic platforms can precisely and efficiently manipulate\, sort\, and process cells to generate indexed sequencing libraries\, allowing for high-throughput single-cell analysis of the genome\, transcriptome\, proteome\, and epigenome. Such technology has been instrumental in the global effort to create a human cell atlas\, with the ambitious goal of identifying and cataloging all human cell types and cell states in health and disease. However\, not all cell phenotypes are directly encoded in the genome and high-throughput sequencing cannot probe the full space of cellular identity. Therefore\, microscopy remains one of the most powerful and versatile tools for characterizing cells. Fluorescent imaging and quantitative non-linear optical imaging can reveal morphological characteristics\, protein localization\, chromatin organization\, and chemical composition in single cells. Both single-cell genomics and microscopy can uncover heterogeneity in cellular populations that would otherwise be obscured in ensemble measurement. In this talk\, I will discuss a suite of new microfluidic platforms for coupling genomic measurements and optical measurements of the same single cell\, and some novel computational approaches to grapple with these new datasets. With a combination of new hardware and software\, our goal is to converge on a quantitative and comprehensive understanding of cellular identity.
URL:https://seasevents.nmsdev7.com/event/be-seminar-5/
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ORGANIZER;CN="Bioengineering":MAILTO:be@seas.upenn.edu
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